新型抗菌涂层Ag2O-TiO2-NTS在治疗人工关节置换术后假体周围感染的体内外研究

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摘要目的：本研究通过体内外实验，探讨装载了纳米氧化银颗粒的钛纳米管(Ag2O-TiO2-NTS) 在治疗人工关节置换术后假体周围感染过程中，对表面葡萄球菌及金黄色葡萄球菌的抑菌作用。方法：体外实验：选取不同浓度的Ag2O-TiO2-NTS溶液与表面葡萄球菌及金黄色葡萄球菌共培养，并计算菌落数及抗菌率。体内实验：选用32只新西兰大白兔建立人工关节置换术后感染模型，并随机分为表-Ag钉组、表-普钉组、金-Ag钉组、金-普钉组。表-Ag钉组、金-Ag钉组兔子术中使用Ag2O-TiO2-NTS复合涂层的螺钉，表-普钉组、金-普钉组使用没有涂层的普通螺钉。拆线后1天，于表-Ag钉组、表-普钉组兔术侧膝关节注入表皮葡萄球菌悬液，于金-Ag钉组、金-普钉组兔术侧膝关节注入金黄色葡萄球菌悬液。在手术当天、注射菌液当天及注射后1、3、5、7、10、14天记录所有大白兔肛温、CRP、ESR及PCT。结果：体外实验：不同浓度的Ag2O-TiO2-NTS溶液及生理盐水两组菌落数及抗菌率两两比较，每组中仅1mmol/L、2mmol/L两组P＞0.05，差异无统计学意义，余两两比较P＜0.01，差异有统计学意义。而两组同一浓度均为P＞0.05，差异无统计学意义。体内实验：组内比较，各时间点肛温、CRP、ESR、PCT差异有统计学意义（P＜0.01），时间与分组有交互作用。组间比较，表-Ag钉组、表-普钉组及金-Ag钉组、金-普钉组的肛温、CRP、ESR、PCT差异有统计学意义（P＜0.01）。LSD-test检验两两比较肛温、CRP、ESR、PCT，4组动物在术前及拆线后注射前1天两两比较，均P＞0.05，差异无统计学意义；表-Ag钉组与表-普钉组比较及金-Ag钉组与金-普钉组比较，在注射后1、3、5、7、10、14天，均为P＜0.01，差异有统计学意义；表-Ag钉组与金-Ag钉组的比较及表-普钉组、金-普钉组的比较，在注射后1、3、5、7、10、14天，均为P＞0.05，差异无统计学意义。结论：装载了纳米氧化银颗粒的钛纳米管(Ag2O-TiO2-NTS)在体内外均对表面葡萄球菌及金黄色葡萄球菌具有良好的抗菌作用，在治疗人工关节置换术后假体周围感染有良好前景。

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关键词 ：装载了纳米氧化银颗粒的钛纳米管，人工关节置换术后假体周围感染，表面葡萄球菌，金黄色葡萄球菌

Abstract：Objective The aim of this study was to determine the effects of novel antibacterial coatings made from composite material titanium oxide nanotubes embedded with silver dioxide nanoparticles (Ag2O-TiO2-NTs) on epidermidis staphylococcus and staphylococcus aureus infections after artifcial joint replacement in vivo and in vitro. Methods In vitro different concentrations of Ag2O-TiO2-NTs solution were co-cultured with epidermidis staphylococcus and staphylococcus aureus, and the colony number and antibacterial rate were calculated. Thirty-two New Zealand rabbits were selected to construct animal models of prosthetic infections. The animal models were randomly divided into 4 groups. Animals in the experimental group were implanted with nails with Ag2O-TiO2-NTs composite coatings, whereas those in the control group were implanted with common nails. After taking out stitches, epidermidis staphylococcus and staphylococcus aureus supernatant (1 mL) were injected into the affected articular cavity of the knee joint of each animal seperately. Anti-infection effects of Ag2O-TiO2-NTs composite coatings were evaluated. These included body temperature, serum C-reactive protein (CRP) concentration, erythrocyte sedimentation rate (ESR) and procalcitonin (PCT). Results Infection rates of prostheses of animals in the experimental group were also lower than the control group (P<0.05).Measured values of body temperature, CRP, ESR, and PCT of animals in the experimental groups (epidermidis staphylococcus and staphylococcus aureus- Ag2O-TiO2-NTs) were remarkably lower than in the control groups, at different time points after bacterial inoculation (all P<0.05). Conclusions Artifcial joint prostheses with novel antibacterial coatings, made from Ag2O-TiO2-NTs composites have better performance regarding anti- epidermidis staphylococcus and staphylococcus aureus infections.

Key words： Titanium oxide nanotubes embedded with silver dioxide nanoparticles, Prosthetic joints infections， Epidermidis staphylococcus，Staphylococcus aureus

收稿日期: 2019-03-27 出版日期: 2019-09-17

基金资助:广州市医药卫生科技项目;广州市番禺区医疗卫生项目

通讯作者: 曾展鹏 E-mail: 21474082@qq.com

引用本文:

曾展鹏. 新型抗菌涂层Ag2O-TiO2-NTS在治疗人工关节置换术后假体周围感染的体内外研究[J]. , 2019, 26(4): 0-0.
Zhan-Peng ZENG. Titanium oxide nanotubes embedded with silver dioxide nanoparticles for treatment of prosthetic joints infections in vivo and in vitro. , 2019, 26(4): 0-0.

链接本文:

http://edit.chinamet.cn/Jweb_jsgncl/CN/ 或 http://edit.chinamet.cn/Jweb_jsgncl/CN/Y2019/V26/I4/0

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